为探索建立不依赖于再生体系的遗传转化体系，突破彩色马蹄莲（Zantedeschia hybrida）遗传转化的技术瓶颈，对黄花马蹄莲‘京彩阳光’（Z. elliottiana ‘Jingcai Yangguang’）快繁技术进行优化，并以块茎切片为侵染对象，以GUS基因为报告基因，通过正交试验设计和PCR鉴定，初步建立其农杆菌（Agrobacterium）介导的遗传转化体系。结果表明：1.0 mg·L^-16-BA和0.8 mg·L^-1 NAA、3.0 mg·L^-1 6-BA、1.0 mg·L^-16-BA和0.5 mg·L^-1 NAA分别为愈伤组织诱导、丛生芽诱导和壮芽的最佳激素组成；表面形成霜状愈伤的块茎切片是农杆菌侵染的合适对象；潮霉素对块茎切片愈伤组织生长有明显抑制作用。菌液OD600值为 0.6、重悬后摇菌2 h、侵染25 min、共培养3 d时，块茎切片的蓝斑率最高（43.3%），其中71.1%再生株系呈阳性。综上，建立的黄花马蹄莲‘京彩阳光’组培快繁体系高效，建立的遗传转化体系具有可行性。

 To explore the establishment of a genetic transformation system independent of regeneration systems and break through the technical bottleneck of genetic transformation in  Zantedeschia hybrida, the rapid propagation technology of Z. elliottiana ‘Jingcai Yangguang’ was optimized, tuber slices were used as infection objects, the GUS gene was used as a reporter gene, and an Agrobacterium-mediated genetic transformation system was preliminarily established through orthogonal experimental design and PCR identification. The results show that 1.0 mg·L^-1 6-BA and 0.8 mg·L^-1 NAA, 3.0 mg·L^-1 6-BA, and 1.0 mg·L^-16-BA and 0.5 mg·L^-1 NAA are the optimal hormone compositions for callus induction, clustered shoot induction, and vigorous shoot formation, respectively; the tuber slices with frost-like callus on the surface are suitable objects for Agrobacterium infection; hygromycin obviously inhibits callus growth of tuber slices. Under the conditions of bacterial suspension OD600 value of 0.6, shaking bacteria for 2 h after resuspension, infection for 25 min, and co-cultivation for 3 d, the blue-staining rate of tuber slices reaches the highest value (43.3%), among which 71.1% of the regenerated lines are positive. In conclusion, the established tissue culture rapid propagation system of Z. elliottiana ‘Jingcai Yangguang’ is efficient, and the established genetic transformation system is feasible.