以栽培于江苏南京的安徽、河南和浙江3个种源的天竺桂（Cinnamomum japonicum Siebold）为研究材料，对自然越冬过程其叶片中激素含量和转录组进行分析。结果表明：自然越冬过程河南种源的天竺桂叶片中吲哚乙酸含量显著高于（p＜0.05）安徽和浙江种源，安徽种源的天竺桂叶片中脱落酸、水杨酸和赤霉素A1含量总体显著高于河南和浙江种源。转录组测序共获得192 466个unigene，进行Nr、Nt、KO、Swiss-Prot、KOG、GO和PFAM 7大数据库的基因功能注释，注释率为54.4%。差异表达基因筛选、k-means聚类分析和加权基因共表达网络分析（WGCNA）结果表明：植物-病原体互作、淀粉和蔗糖代谢以及植物激素信号转导3个关键KEGG通路显著富集。脱落酸和水杨酸信号通路是天竺桂应对自然越冬的重要代谢通路，其中脱落酸受体基因（PYR/PYL）、蔗糖非发酵激酶基因（SnRK2）、脱落酸应答元件结合因子基因（ABF）、病程相关基因非表达子1（NPR1）、TGACG结合模体（TGA）转录因子基因和病程相关基因（PR-1）具有重要作用。随机挑选6个差异表达基因的实时荧光定量PCR相对表达量与转录组表达量的变化趋势一致。综上所述，3个种源天竺桂通过特异的激素动态平衡与激素信号通路协同调控适应自然越冬，其中河南种源表现出更强的抗寒代谢调控能力。

 Taking three Cinnamomum japonicum Siebold provenances from Anhui, Henan, and Zhejiang, which cultivated in Nanjing of Jiangsu were selected as research materials, the hormone content and transcriptome of their leaves during natural overwintering process were analyzed. The results indicate that the indole-3-acetic acid content in the leaves of  C. japonicum from Henan provenance is significantly higher (p<0.05) than that of Anhui and Zhejiang provenances, moreover the abscisic acid, salicylic acid, and gibberellin A1 contents in the leaves of  C. japonicum from Anhui provenance are significantly higher than those of Henan and Zhejiang provenances during natural overwintering process in general. A total of 192 466 unigenes obtained from transcriptome sequencing are performed functional annotation using seven major databases, including Nr, Nt, KO, Swiss-Prot, KOG, GO, and PFAM, achieving an annotation rate of 54.4%. The results of differentially expressed gene screening, k-means clustering analysis, and weighted gene co-expression network analysis (WGCNA) indicate that three key KEGG pathways of plant-pathogen interaction, starch and sucrose metabolism, and plant hormone signal transduction are significantly enriched. The abscisic acid and salicylic acid signaling pathways are important metabolic pathways for C. japonicum to cope with natural overwintering. Among them, abscisic acid receptor gene (PYR/PYL), sucrose non-fermenting kinase gene (SnRK2), abscisic acid-responsive element binding factor  gene (ABF), non-expressor  of pathogenesis-related gene 1 (NPR1), TGACG motif-binding (TGA) transcription factor gene, and pathogenesis-related gene (PR-1) play important roles. The relative expression levels of six randomly selected differentially expressed genes measured by real-time fluorescence quantitative PCR are consistent with the trends of transcriptome expression levels. In summary, three provenances of C. japonicum adapt to natural overwintering through a coordinated regulation of specific hormone dynamic balance and hormone signaling pathways, with the Henan provenance exhibiting a stronger capacity for cold resistance metabolic regulation.