为探究抗虫水稻（Oryza sativa Linn.）响应褐飞虱〔Nilaparvata lugens (St-l)〕取食的生理变化机制，以抗虫水稻PR6和感虫水稻品种‘TN1’为材料，测定并分析了接褐飞虱后植株生长指标，茎秆中细胞壁成分、糖类、游离氨基酸含量，相关酶活性及基因表达的变化。结果表明：PR6的抗褐飞虱级别为3，抗性水平为抗虫，并具有持抗性。褐飞虱取食后，PR6的地上部和茎秆鲜质量均降低，但降幅明显小于‘TN1’，而其叶片鲜质量无显著变化；PR6茎秆中木质素含量极其显著（P≤0.001）升高，纤维素和总果胶含量则极其显著降低，但三者含量均高于‘TN1’；PR6茎秆中总糖、可溶性糖、淀粉和蔗糖含量的降幅均明显小于‘TN1’，且总糖和淀粉含量极其显著或极显著（P≤0.01）高于‘TN1’。褐飞虱取食后，PR6茎秆中总游离氨基酸含量极显著降低，而‘TN1’茎秆中总游离氨基酸含量极其显著升高；另外，PR6茎秆中纤维素酶、β-葡萄糖苷酶、果胶甲酯酶及α-淀粉酶的活性极其显著或显著（P≤0.05）低于‘TN1’。实时荧光定量PCR分析结果表明：褐飞虱取食后，PR6和‘TN1’茎秆中纤维素酶基因、糖基转移酶基因、果胶裂解酶基因及α-淀粉酶基因的表达随取食时间延长整体表现为先升后降的变化趋势，PR6茎秆中木质素合成相关基因的表达整体上持续上调。综合分析表明：PR6具有抗褐飞虱特性，褐飞虱取食后抗虫水稻PR6的生理变化与感虫水稻‘TN1’有明显差异，且抗虫水稻PR6茎秆中木质素合成相关基因的表达受褐飞虱取食诱导较感虫水稻‘TN1’更明显。

To investigate the physiological variation  mechanism of insect-resistant rice (Oryza sativa Linn.) responding to brown planthopper ［Nilaparvata lugens (St-l)］ feeding, the insect-resistant rice PR6 and insect-susceptible rice cultivar ‘TN1’ were taken as materials, and the variations of plant growth indexes, contents of cell wall components carbohydrates and free amino acids, and activities and gene expression of related enzymes in stems after brown planthopper inoculation were determined and analyzed. The results show that PR6 exhibits a brown planthopper resistance level of grade 3, and the insect resistance level is insect-resistant with persistent resistance. After brown planthopper feeding, both above-ground part and stem fresh masses of PR6 decrease, but the decrements are evidently smaller than ‘TN1’, while its leaf fresh mass shows no significant variations; lignin content in PR6 stems increases greatly extremely significantly (P≤0.001), contents of cellulose and total pectin decrease greatly extremely significantly, but the contents of above three components are all higher than ‘TN1’; the decrements in contents of total carbohydrates, soluble sugar, starch, and sucrose in PR6 stems are obviously smaller than ‘TN1’, and contents of total carbohydrates and starch are greatly extremely significantly or extremely significantly (P≤0.01) higher than ‘TN1’. After brown planthopper feeding, the total free amino acid content extremely significantly decreases in PR6 stems, but greatly  extremely significantly increases in ‘TN1’ stems; additionally, activities of cellulase, β-glucosidase, pectinesterase, and α-amylase in PR6 stems are greatly extremely significantly or significantly (P≤0.05) lower than  ‘TN1’. The real-time quantitative PCR analysis results show that after brown planthopper feeding, the expressions of cellulase genes, glycosyltransferase genes, pectate lyase genes, and α-amylase genes in both PR6 and ‘TN1’ stems generally show a variation tendency of first increase and then decrease with the elongation of feeding time, while lignin biosynthesis-related genes in PR6 stems are continuously up-regulated in general. The comprehensive analysis indicates that PR6 possesses brown planthopper resistant characteristics, the physiological variations of insect-resistant rice PR6 are obviously different from insect-susceptible rice ‘TN1’ after brown planthopper feeding, and the expression of lignin biosynthesis-related genes induced by brown planthopper feeding in insectresistant rice PR6 stems  is more pronounced than insect-susceptible rice ‘TN1’.