| 摘要 | 采用高效液相色谱技术,分析不同波段和辐照度紫外线短时间(10 min)照射对采后人参(Panax ginseng C. A. Meyer)根中7种人参皂苷含量的影响。结果表明:与对照相比较,9.600 W·m-2 UV-A和0.214 W·m-2 UV-B处理均能显著提高(P<0.05)人参皂苷总含量(分别为9.692和9.006 mg·g-1),其中9.600 W·m-2 UV-A处理的效果最为突出,增幅达39.31%。0.055和0.161 W·m-2UV-B处理则显著降低了人参皂苷总含量。在单体人参皂苷方面,9.600 W·m-2 UV-A和0.214 W·m-2 UV-B处理显著提高了人参皂苷Rb1和Re含量,而人参皂苷Rc含量在所有紫外线处理后均有不同程度的下降。0.161 W·m-2 UV-B处理较对照显著增加了原人参二醇型人参皂苷含量与原人参三醇型人参皂苷含量的比值,增幅达101.37%。研究结果表明:9.600 W·m-2 UV-A和0.214 W·m-2 UV-B短时间照射均可显著提升采后人参根中人参皂苷总含量及人参皂苷Rb1和Re的含量,是一种有效的人参采后品质调控手段。
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| Abstract | The effects of short-term (10 min) ultraviolet irradiation with different wavelengths and irradiances on the contents of seven ginsenosides in postharvest Panax ginseng C. A. Meyer roots were analyzed by using high-performance liquid chromatography. The results show that compared with the control, both 9.600 W·m-2 UV-A and 0.214 W·m-2 UV-B treatments can significantly increase (P<0.05) the total ginsenoside content (which are 9.692 and 9.006 mg·g-1, respectively), among which the 9.600 W·m-2 UV-A treatment is the most effective, showing an increment of 39.31%. In contrast, 0.055 and 0.161 W·m-2 UV-B treatments significantly reduce the total ginsenoside content. Regarding individual ginsenosides, 9.600 W·m-2 UV-A and 0.214 W·m-2 UV-B treatments significantly increase the contents of ginsenosides Rb1 and Re, while ginsenoside Rc content decreases to different degrees after all ultraviolet treatments. The 0.161 W·m-2 UV-B treatment significantly increases the ratio of protopanaxadiol-type ginsenoside content to protopanaxatriol-type ginsenoside content compared with the control, with an increment of 101.37%. It is suggested that short-term irradiation with 9.600 W·m-2 UV-A and 0.214 W·m-2 UV-B can both significantly elevate the total ginsenoside content as well as the contents of ginsenosides Rb1 and Re in postharvest P. ginseng roots, representing an effective method for postharvest quality regulation of P. ginseng.
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| 关键词 | 人参; 紫外线; 采后照射; 辐照度; 人参皂苷 |
| Key words | Panax ginseng C. A. Meyer; ultraviolet ray; postharvest irradiation; irradiance; ginsenoside |
| 作者 | 王紫嫣1,2, 赵兴增2, 谭文玥1,2, 柳成航2, 许梦洋2, 杜建科2, 贾晓东1,2 |
| 所在单位 | 1. 南京中医药大学, 江苏 南京 210023;2. 江苏省中国科学院植物研究所(南京中山植物园) 江苏省植物资源保护与利用重点实验室, 江苏 南京 210014 |
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| 基金项目 | 国家自然科学基金项目(32471918; 32401644); 江苏省中国科学院植物研究所博士人才科研启动基金项目(JIBTF202508); 江苏省林业科技创新与推广重大项目(LYKJ[2025]07) |