为探究PAL基因家族在越南参（Panax vietnamensis Ha et Grushv.）中的功能，通过同源比对和隐马尔可夫模型检索对PAL基因家族成员进行鉴定，对其相关的理化性质、系统发育关系、基因结构、启动子顺式作用元件、共线性特征、组织特异性表达及其在水杨酸处理下的表达模式进行了系统解析，并在越南参愈伤组织中对PvPAL2和PvPAL4进行了功能验证。结果显示：在越南参中共鉴定出4个PAL基因，依据其染色体位置分别将命名为PvPAL1、PvPAL2、PvPAL3、PvPAL4。4个PvPAL蛋白的氨基酸数为712~719，相对分子质量为77 732.84~78 236.50，理论等电点为pI 6.00至pI 6.24。越南参PvPAL2与PvPAL4聚在同一个分支，亲缘关系较近，且PvPAL2与PvPAL4具有相似的外显子和内含子结构。PvPAL启动子区含有大量顺式作用元件，其中，非生物和生物胁迫响应元件数量最多。总体上看，PvPAL在大多数组织间的表达水平差异显著(P<0.05)。水杨酸处理6 h后，PvPAL1、PvPAL2和PvPAL4表达受到显著诱导。此外，愈伤组织瞬时过表达显示PvPAL2与PvPAL4可促进木质素和类黄酮积累。综上所述，4个PvPAL基因整体保守性较高，且基因表达水平显著受水杨酸诱导，PvPAL2和PvPAL4显著促进了木质素和类黄酮的积累，在越南参生长发育中发挥重要作用。

 To investigate the functions of the PAL gene family in Panax vietnamensis Ha et Grushv., PAL gene family members were identified through homology alignment and Hidden Markov Model searches, their related physicochemical properties, phylogenetic relationships, gene structures, promoter cis-acting elements, collinearity characteristics, tissue-specific expressions, and expression profiles under salicylic acid treatment were systematically analyzed, and functional validation of PvPAL2 and PvPAL4 was performed in P. vietnamensis callus. The results show that four PAL genes are identified in P. vietnamensis in total, and named PvPAL1, PvPAL2, PvPAL3, and PvPAL4, respectively based on their chromosomal locations. The amino acid numbers of four PvPAL proteins are 712-719, their relative molecular masses are 77 732.84-78 236.50, and their theoretical isoelectric points are pI 6.00 to pI 6.24. PvPAL2 and PvPAL4 are clustered into the same branch, indicating a close genetic relationship, and PvPAL2 and PvPAL4 possess similar exon and intron structures. The promoter regions of PvPAL contain numerous cis-acting elements, among which, the number of abiotic and biotic stress response elements is the most. Overall, the expression levels of PvPAL differ significantly (P<0.05) among most tissues. After 6 h of salicylic acid treatment, the expressions of PvPAL1, PvPAL2, and PvPAL4 are significantly induced. Furthermore, the transient overexpression in callus demonstrates that PvPAL2 and PvPAL4 can promote the accumulation of lignin and flavonoid. In conclusion, the four PvPAL genes exhibit relatively high conservation overall, their expression levels are significantly induced by salicylic acid, and PvPAL2 and PvPAL4 significantly promote the accumulation of lignin and flavonoid, playing important roles in the growth and development of P. vietnamensis.