摘要 | 以落羽杉属(Taxodium Rich.)22个人工杂交组合F1代实生苗中筛选获得的76株个体为材料,系统评估24个表型性状变异、遗传多样性、相关性、亲本组合效应及表型类群分化。结果显示:落羽杉属杂交子代叶排列方式、叶形、侧生小枝生长朝向和侧生小枝排列方式的变异系数较大(大于106%),可塑性强;树高、冠形、秋季叶色(11月中旬)的Shannon-Wiener指数较高(分别为1.94、1.58、1.54),分化明显。相关性分析结果表明:侧生小枝宽度、叶夹角、叶长间呈极强正相关,而三者与树高呈强负相关,反映资源分配权衡;秋季叶色(11月中旬)和冬季叶色(12月中旬)与落叶期呈中等负相关,揭示二者生理关联。亲本组合效应分析结果显示:墨西哥落羽杉(T. mucronatum Ten.) × 池杉〔T. distichum var. imbricatum (Nutt.) Croom〕的子代在树高、胸径、落叶期、叶形、叶密度、叶排列方式及赤枯病抗性上表现优异,而回交组合中山杉(T. ‘Zhongshanshan’) × 墨西哥落羽杉的子代普遍表现劣势,落羽杉〔T. distichum (Linn.) Rich.〕亲本在秋冬季叶色上起主导作用。聚类分析将76个子代个体划分为4个类群,其中类群2(综合性状优异型)为多目标育种核心类群;类群4(均衡观赏型)冠形及秋冬季叶色俱佳,适用于景观选育;类群3(高大晚落叶型)规模较大,胸径生长突出,可作为速生材料补充来源。综上,在落羽杉属杂交育种中,应优先选择遗传距离较远的亲本进行杂交,以最大化杂种优势。在优良类群早期筛选阶段,可在优先筛选类群2的基础上,进一步从类群3中选拔综合表现较优的子代个体作为补充材料。
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Abstract | Taking 76 individuals selected from the F1 progeny seedlings of 22 artificial hybrid combinations of Taxodium Rich. as materials, the variation, genetic diversity, correlations, parental combination effects, and phenotypic group differentiation of 24 phenotypic traits were systematically evaluated. The results show that the coefficients of variation for leaf arrangement, leaf shape, lateral branch orientation, and lateral branch arrangement in Taxodium hybrid progenies are relatively large (greater than 106%), indicating strong plasticity; the Shannon-Wiener indexes of tree height, crown shape, and leaf color in autumn (mid-November) are relatively high (which are 1.94, 1.58, and 1.54, respectively), indicating evident differentiation. The correlation analysis result shows that lateral branch width, leaf angle, and leaf length exhibit extremely strong positive correlations, but they all show strong negative correlations with tree height, reflecting resource allocation trade-offs; leaf color in autumn (mid-November) and winter (mid-December) show moderate negative correlations with defoliation stage, revealing their physiological associations. The parental combination effect analysis result shows that the progenies of T. mucronatum Ten. × T. distichum var. imbricatum (Nutt.) Croom exhibit superior performance in tree height, diameter at breast height, defoliation stage, leaf shape, leaf density, leaf arrangement, and red blight resistance, while the progenies of the backcross combination T. ‘Zhongshanshan’ × T. mucronatum generally show inferior performance, and T. distichum (Linn.) Rich. parents play a dominant role in leaf color in autumn and winter. The cluster analysis divides the 76 progeny individuals into four groups, among which group 2 (excellent comprehensive trait type) is identified as the core group for multi-objective breeding; group 4 (balanced ornamental type) exhibits excellent crown shape and leaf color in autumn and winter, suitable for landscape selection; group 3 (tall and late-defoliation type) has a larger scale and outstanding diameter at breast height growth, which could serve as a supplementary source of fast-growing materials. In conclusion, in Taxodium hybrid breeding, parents with greater genetic distance should be preferentially selected for hybridization to maximize heterosis. In the early selection stage of superior groups, on the basis of prioritizing group 2, individuals with excellent comprehensive performance from group 3 could be further selected as supplementary materials. |
| 关键词 | 落羽杉属; 杂交子代; 遗传多样性; 亲本组合效应; 聚类分析; 早期筛选 |
| Key words | Taxodium Rich.; hybrid progeny; genetic diversity; parental combination effect; cluster analysis; early selection |
| 作者 | 尤慧, 王紫阳, 李海滨, 杨颖, 於朝广 |
| 所在单位 | 江苏省中国科学院植物研究所(南京中山植物园) 江苏省植物分类与资源保护利用重点实验室, 江苏 南京 210014 |
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| 基金项目 | 中央林业草原改革发展资金项目(江苏省落羽杉国家林木种质资源库) |