Total extracts of 80% （volume fraction） ethanol （hereinafter referred to as total extracts） and its dichloromethane， ethyl acetate， and water fractions were obtained from leaves and fruits of four Evodia J. R. et G. Forst. species by using reflux extraction and solvent extraction， and their in vitro antitumor activities and toxicities were evaluated by using MTT method. The results show that the extraction rates of total extracts from leaves and fruits of E. ailanthifolia Pierre， E. austrosinensis Hand.-Mazz.， E. daniellii （Benn.） Hemsl.， and E. delavayi Dode are in the range of 13.00%-30.35%. The pharmacological experiment result shows that at the drug concentration of 10 μg·mL^-1， the inhibition rates of total extracts and its water fraction from leaves of E. ailanthifolia， ethyl acetate fraction of total extracts from fruits of E. austrosinensis， and total extracts from fruits of E. daniellii on breast cancer cell MDA-MB-231 and hepatoma cell HepG2 are higher than 50%， those of dichloromethane fraction of total extracts from leaves of E. ailanthifolia on three tumor cells are all higher than 50%， those of ethyl acetate fraction of total extracts from leaves of E. ailanthifolia on breast cancer cell MDA-MB-231 and colon cancer cell LoVo are higher than 50%， and their inhibition rates on the normal human aortic smooth muscle cell HASMC are lower than 40%. Therefore， above extracts have potential values as a source of natural antitumor drug.