The chemical constituents in the methanol extract of Prunus domestica Linn. branches were isolated and identified by comprehensively employing multiple column chromatography and semi-preparative high-performance liquid chromatography technologies combined with spectral methods such as nuclear magnetic resonance and mass spectrometry. Ten compounds are identified, namely fraxinol-6-β-D-galacto-pyranoside (1), (+)-catechin (2), afzelechin (3), (-)-epicatechin (4), prunasin (5), (-)-annphenone (6), (1-p-hydroxy-cis-cinnamoyl) cinnamic acid (7), naringenin (8), proanthocyanidin A-2 (9), and 4-O-β-D-glucopyranosyl-2-hydroxy-6-methoxyacetophenone (10). All the above compounds are isolated from P. domestica for the first time.