Contents of rutin， isoquercitrin， kaempferol-3-O-rutinoside， neochlorogenic acid， chlorogenic acid， 3，5-dicaffeoylquinic acid and 4，5-dicaffeoylquinic acid in root， stem and leaf of Gynura bicolor （Willd.） DC. were determined by using HPLC method. The results show that all 7 components can be detected in leaf of G. bicolor， and their contents are 0.279-4.804 mg·g^-1， in which， contents of chlorogenic acid and isoquercitrin are relatively high， while contents of rutin and neochlorogenic acid are relatively low； only chlorogenic acid， 3，5-dicaffeoylquinic acid and 4，5-dicaffeoylquinic acid are detected in its root and stem， and their contents are all significantly lower than those in leaf. It is recommended that leaf of G. bicolor can be used as the medicinal part based on the measured result.