Total flavonoids content in leaf of wild Lycium barbarum Linn. from June to October at 15 locations from Haidong and Haixi districts in Qinghai Province was determined. The results show that there are obvious differences in total flavonoids content in leaf of wild L. barbarum at different locations and collection times. Total flavonoids content in leaf from 15 locations is 0. 127% -3. 443%, in which that from county town and Xiangride Town in Dulan County reaches 3. 443% and 2. 582%, respectively. Total flavonoids content in leaf from county town in Dulan County decreases gradually with delaying of collection time, that is higher in June and July (3. 443% and 3. 382%, respectively) and is the lowest in October (only 1. 479%). The comprehensive analysis result shows that total flavonoids content in leaf of wild L. barbarum from Haixi District in Qinghai Province is higher, and the best collection time is June and July .