2026年4月12日 星期日
落羽杉×墨西哥落羽杉种子发育过程中部分内源激素和主要营养成分含量变化
Variations of contents of several endogenous hormones and major nutrients during the seed development process of Taxodium distichum × Taxodium mucronatum
2026年 第35卷 第2期 页码[104-107]    下载全文[0.9MB]  
摘要

为探明落羽杉属(Taxodium Rich.)种间杂交种子发育过程中内源激素和营养成分的变化规律,以6个发育阶段的落羽杉〔T. distichum (Linn.) Rich.〕×墨西哥落羽杉(T. mucronatum Ten.)种子为材料,对吲哚乙酸(IAA)、异戊烯腺嘌呤核苷(iPA)、赤霉素4(GA4)、脱落酸(ABA)、茉莉酸(JA)、水杨酸(SA)以及可溶性糖、可溶性淀粉、可溶性蛋白质的含量变化进行了分析。结果表明:内源激素含量随种子发育进程呈现不同的变化格局,IAA和ABA含量维持在较高水平,而iPA含量则处于较低水平。比较来看,IAA含量在子叶胚前期最高,iPA和GA4含量在子叶胚期最高,JA含量在柱状胚期最高,ABA含量在柱状胚期和子叶胚前期较高,SA含量在显性胚期至子叶胚前期较高。可溶性糖、可溶性淀粉和可溶性蛋白质含量总体呈升高趋势,且在子叶胚期最高。研究结果显示:IAA和ABA在落羽杉×墨西哥落羽杉种子发育过程中发挥主要调控作用,可溶性糖、可溶性淀粉和可溶性蛋白质随着种子发育不断积累。

Abstract

To investigate the variation patterns of endogenous hormones and nutrients during the seed development process of interspecific hybrids of Taxodium Rich., taking seeds of T. distichum (Linn.) Rich. × T. mucronatum Ten. at six developmental stages as materials, the variations of contents of  indole-3-acetic acid (IAA), isopentenyladenosine (iPA), gibberellin 4 (GA4), abscisic acid (ABA), jasmonic acid (JA) and salicylic acid (SA), as well as  soluble sugar, soluble starch and soluble protein were analyzed. The results indicate that as the seeds develop, the contents of endogenous hormones exhibit different variation patterns, IAA and ABA contents maintain at a relatively high level, while iPA content maintains at a relatively low level. In comparison, IAA content reaches the highest value at the pre-cotyledonary embryo stage, iPA and GA4 contents reach the highest value at the cotyledonary embryo stage, JA content reaches the highest value at the columnar embryo stage, ABA content is at a relatively high level at both the columnar embryo stage and pre-cotyledonary embryo stage, SA content is at a high level from the dominant embryo stage to the pre-cotyledonary embryo stage. The contents of soluble sugar, soluble starch and soluble protein generally exhibit an increasing trend, with the highest level at the cotyledonary embryo stage. The results reveal that IAA and ABA play a major regulatory role during the seed development of T. distichum × T. mucronatum, and soluble sugar, soluble starch and soluble protein continuously accumulate with the development of seeds.

 

关键词落羽杉属; 种子发育; 内源激素; 营养成分
Key wordsTaxodium Rich.; seed development; endogenous hormone; nutrient
作者王紫阳, 杨颖, 华建峰, 殷云龙, 於朝广
所在单位江苏省中国科学院植物研究所(南京中山植物园) 江苏省植物资源保护与利用重点实验室, 江苏 南京 210014
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基金项目江苏落羽杉属树木育种及培育省级长期科研基地项目〔LYKJ(2021)05〕; 2025年江苏省落羽杉国家林木种质资源库项目(362502317)